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KMID : 0043319860090010029
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Archives of Pharmacal Research
1986 Volume.9 No. 1 p.29 ~ p.38
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Effect of Ginseng Saponin on the Na+, K+-ATPase of Dog Cardiac Sarcolemma
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Lee SW
Lee JS/Kim YH/Jin KD
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Abstract
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The effects of ginseng saponins on the sarcolemmal Na+, K+-ATPase were compared to gypsophila saponin, sodium dodecylsulfate (SDS), and Triton X-100 to elucidate whether the effects are due to the membrane disruption, using a highly enriched preparation of cardiac sarcolemma prepared from dog ventricular myocardium. About 26% and 29% of vesicles in the preparation, enriched in ouabain-sensitive Na+, K+-ATPase, beta-adrenergic and muscarinic receptors are rightside-out and inside-out in orientation, respectively. Ginseng saponins (triol>total>diol) inhibited Na+, K+-ATPase activity, Na+-dependent phosphorylation and [3H]ouabain binding of sarcolemmal vesicles. However, gypsophila saponin, SDS (0.4mcg/mcg protein) and Triton X-100 (0.6mcg/mcg protein) caused about 1.35 and 1.40-fold increase in Na+, K+-ATPase activity and [3H] ouabain binding, respectively. Especially, the activating effect of gypsophila saponin on membrane Na+, K+-ATPase was detected at gypsophila saponin to sarcolemmal protein ratios as high as 100. Low dose of ginseng saponin (3mcg/mcg protein) decreased the phosphorylation sites and the concentration of ouabain binding sites (Bmax) without affecting the turnover number and affinity for ouabain binding, while gypsophila saponin, SDS (0.4mcg/mcg protein), and Triton X-100 (0.6mcg/mcg protein) increased the Bmax. The results suggest that ginseng saponins cause a decrease in the number of active sites by interacting directly with Na+, K+-ATPase before disruption of membrane barriers of sarcolemmal vesicles.
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KEYWORD
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